“The therapeutic success of different boron neutron capture therapy (BNCT) protocols employing the hamster cheek pouch oral cancer model has been previously reported by our laboratory (see also <http://www.newsrx.com/library/topics/Oral-Cancer.html> Oral Cancer). The aim of this study was to explore potential mechanisms of BNCT-induced damage to tumor in terms of potential inhibition in DNA synthesis and induction of apoptosis in the tumors that underwent partial remission following application of the different BNCT protocols in this model,” researchers in Argentina report.

“We evaluated DNA synthesis employing incorporation of 5-bromo-2′-deoxyuridine as an end-point. Apoptosis was evaluated by immunohistochemistry employing the deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling technique and Bax and Bcl-2 labeling. These studies were performed in tumors that underwent partial remission 1-30 days post-BNCT mediated by boronophenylalanine (BPA), GB-10 ((Na2B10H10)-B-10) or (BPA + GB-10). BNCT exerted a marked inhibitory effect on DNA synthesis in tumors for all the protocols under study. The inhibitory effect of BPA-BNCT occurred as soon as 1 day post-treatment (P < 0.001). Conversely, the effect of GB-10-BNCT became apparent 7-14 days after therapy (P < 0.001) and was sustained until killed at 30 days post-treatment (P < 0.001). (GB-10 + BPA)-BNCT exerted a rapid and persistent effect, conceivably because of the combined effect of BNCT mediated by both boron compounds. The apoptosis studies did not show differences between the pre-treatment group and any of the BNCT groups. One of the mechanisms involved in BNCT-induced tumor control in our model would be an inhibitory effect on DNA synthesis,” wrote R.F. Aromando and colleagues.

The researchers concluded: “Apoptosis does not seem to have a significant role in BNCT-induced tumor control in our model.”

Aromando and colleagues published their study in the Journal of Oral Pathology & Medicine (Insight into the mechanisms underlying tumor response to boron neutron capture therapy in the hamster cheek pouch oral cancer model. Journal of Oral Pathology & Medicine, 2009;38(5):448-454).

For additional information, contact R.F. Aromando, Faculty Dental Oral Pathology, Marcelo T de Alvear 2142, RA-1117 Capital Fed Buenos Aires, Argentina.

Publisher contact information for the Journal of Oral Pathology & Medicine is: Wiley-Blackwell Publishing, Inc., Commerce Place, 350 Main St., Malden 02148, MA, USA.

Keywords: Argentina, Apoptosis, Biotin, DNA, Dietary Supplement, Enzyme Research, Micronutrient, Mouth Neoplasms, Oncology, Oral Cancers, Oral Pathology, Therapy, Transferase, Treatment.

oral cancer research

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